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evaluation of genetic stability in cryopreserved solanum pdf

Genetic stability of wild pear (Pyrus pyraster Burgsd. PDF Questions often arise concerning the genetic stability of plant materials stored in liquid nitrogen for long time periods. This study examined the genetic stability of cryopreserved shoot, Nov 01, 2001В В· Read "Genetic fidelity and viability of Anigozanthos viridis following tissue culture, cold storage and cryopreservation, Plant Science" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips..

Genetic evaluation of mesenchymal stem cells

Evaluation of genetic stability in cryopreserved Solanum. [16] K. Harding, Molecular stability of the ribosomal RNA genes in Solanum tuberosum plants recovered from slow growth and We wish to thank Robyn Taylor for her generous cryopreservation, Euphytica 55 (1991) 141 – 146. help and direction in sample preparation and [17] K. Harding, The methylation status of DNA derived from processing., valuable genetic resources for breeding programs. From our knowledge, in spite of their commercial value, there are no initiatives focused on the ex situ conservation of these cultivars in seed banks. Moreover, this is the first report using sequencing of LCY-B gene as a tool for genetic stability evaluation following liquid nitrogen exposure. 2..

Jan 15, 2011 · Genetic stability in chrysanthemum (cultivar ‘Pasodoble’) apices was studied at each step of an encapsulation–dehydration cryopreservation protocol: control shoots (A), nodal segments after cold treatment (N), apices after osmotic stress (0.3 M sucrose) and cold treatment (P), encapsulation and culture in 0.8 M sucrose (S), dehydration (D), and Apr 03, 2014 · Genetic evaluation of mesenchymal stem cells by G-banded karyotyping in a Cell Technology Center. since the maintenance of a normal karyotype is a reliable indicator of genetic stability of MSCs13 and must be considered as a release criterion for the clinical use of these cells.14.

JJBS Volume 10, Number 1,March 2017 ISSN 1995-6673 Pages 19 - 28 Jordan Journal of Biological Sciences Cryopreservation of Thymbra spicata L. var. spicata and Genetic Stability Assessment of the Cryopreserved Shoot Tips after In the present investigation, the genetic stability of potato (Solanum tuberosum L.) plantlets of the cultivars Agria and Marphona stored under cryopreservation and non-cryopreservation conditions

The use of mesenchymal stem cells/mesenchymal stromal cells (MSCs) in regenerative medicine is increasing throughout the world. These cells can easily be isolated from different tissues and expanded in vitro to produce a sufficient number of cells that do not have infusion-related toxicity in humans ASSESSING GENETIC STABILITY OF A RANGE OF TERRESTRIAL Genetic integrity in cryopreserved microalgae was assessed using a very sensitive molecular fingerprinting technique, AFLP, on 28 terrestrial microalgal strains. In about half of all investigated strains the AFLP fingerprints protocol evaluation and their validation.

JJBS Volume 10, Number 1,March 2017 ISSN 1995-6673 Pages 19 - 28 Jordan Journal of Biological Sciences Cryopreservation of Thymbra spicata L. var. spicata and Genetic Stability Assessment of the Cryopreserved Shoot Tips after Nov 15, 2013В В· Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum .

Attribute Name Values; Creator: Castillo, Nina Rosa F. Abstract: Two studies used DNA markers to assess their usefulness in germplasm identification and evaluation of genetic stability in four cryopreserved Rubus accessions that were stored for over 10 years in liquid nitrogen. Cryopreservation did not affect the plant regeneration potential of A. glutinosa through somatic embryogenesis. The genetic stability of the regenerated material was assessed by flow cytometry. Analysis of DNA ploidy stability of the control, PVS2 treated, cryopreserved somatic embryos and plantlets showed no significant differences. URI:

Oct 01, 2008В В· Summary. We describe an encapsulation and dehydration procedure for the cryopreservation of cork oak (Quercus suber L.) somatic embryos that resulted in at least 90% survival.Genetic stability of the regenerated material was assessed by flow cytometry (FCM), amplified fragment length polymorphisms (AFLP) and simple sequence repeats (SSR). Abstract. Questions often arise concerning the genetic stability of plant materials stored in liquid nitrogen for long time periods. This study examined the genetic stability of cryopreserved shoot tips of Rubus germplasm that were stored in liquid nitrogen for more than 12 yr, then rewarmed and regrown.

Dixit S, Mandal BB, Ahuja S, Srivastava PS (2003) Genetic stability assessment of plants regenerated from cryopreserved embryogenic tissues of Dioscorea Bulbifera L. using RAPD, biochemical and morphological analysis. CryoLetters 24:77–84 PubMed Google Scholar GENOMIC OPPORTUNITIES. Plant cryobiology is a research discipline that is beginning to enter into the genomics arena. Traditionally, the discipline has been considered an extension of the cold hardiness and drought tolerance fields, centered primarily on long term preservation of seeds, shoot tips, dormant buds, pollen, embryos, cell lines, and other propagule types.

Dixit S, Mandal BB, Ahuja S, Srivastava PS (2003) Genetic stability assessment of plants regenerated from cryopreserved embryogenic tissues of Dioscorea Bulbifera L. using RAPD, biochemical and morphological analysis. CryoLetters 24:77–84 PubMed Google Scholar Nov 01, 2001 · Read "Genetic fidelity and viability of Anigozanthos viridis following tissue culture, cold storage and cryopreservation, Plant Science" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

Nov 01, 2001В В· Read "Genetic fidelity and viability of Anigozanthos viridis following tissue culture, cold storage and cryopreservation, Plant Science" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Oct 01, 2008В В· Summary. We describe an encapsulation and dehydration procedure for the cryopreservation of cork oak (Quercus suber L.) somatic embryos that resulted in at least 90% survival.Genetic stability of the regenerated material was assessed by flow cytometry (FCM), amplified fragment length polymorphisms (AFLP) and simple sequence repeats (SSR).

Attribute Name Values; Creator: Castillo, Nina Rosa F. Abstract: Two studies used DNA markers to assess their usefulness in germplasm identification and evaluation of genetic stability in four cryopreserved Rubus accessions that were stored for over 10 years in liquid nitrogen. The use of mesenchymal stem cells/mesenchymal stromal cells (MSCs) in regenerative medicine is increasing throughout the world. These cells can easily be isolated from different tissues and expanded in vitro to produce a sufficient number of cells that do not have infusion-related toxicity in humans

The aim of this study was to compare the genetic stability of chrysanthemum (cv. Pasodoble) apices cryopreserved using two different methods: encapsulation-dehydration and vitrification. These methods were chosen as they are widely used, their efficiency has been reported in several chrysanthemum cultivars, and Me 2 SO is used in one of them. Genetic and epigenetic stability of cryopreserved and cold-stored hops (Humulus lupulus L.)q Elena L. Peredoa,*, Rosa Arroyo-GarcГ­ab, Barbara M. Reedc, M. ГЃngeles Revillaa a Department of Plant Physiology, Universidad de Oviedo, CatedrГЎtico Rodrigo UrГ­a s/n, 33071 Oviedo, Spain bDepartamento de BiotecnologГ­a, Instituto Nacional de InvestigaciГіn Agraria y Alimentaria, Carretera de A

Genetic Stability of Cryopreserved Grapevine (Vitis vinifera L.) Genome by Vitrification Method 382 month in LN (T7). After each treatment, freezing buds were thawed in a water-bath at 38 °C for 3 min. Evaluation of genetic stability in cryopreserved give us a preliminary information on the genetic prunus. In Vitro Cell Developmental Biology Plant 38: stability of the wild pear cryopreserved material 493–500. and let us to be conident of the possibility of using Kawiak, A. & Lojkowska, E. 2004.

valuable genetic resources for breeding programs. From our knowledge, in spite of their commercial value, there are no initiatives focused on the ex situ conservation of these cultivars in seed banks. Moreover, this is the first report using sequencing of LCY-B gene as a tool for genetic stability evaluation following liquid nitrogen exposure. 2. Abstract. Graduation date: 2007Two studies used DNA markers to assess their usefulness in germplasm\ud identification and evaluation of genetic stability in four cryopreserved Rubus\ud accessions that were stored for over 10 years in liquid nitrogen.

The genetic stability of in vitro propagated potato microtubers was assessed using random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. Microtubers were developed through in vitro from potato microplants using standardized protocols. Genetic stability of the in vitro regenerated plants was also assessed by flow cytometry and the directed amplification of minisatellite-region (DAMD) and inter-simple sequence repeat (ISSR) markers.

the genetic stability of potato (Solanum tuberosum) in vitro plants regenerated from apices cryopreserved either by the slow freezing procedure or by the encapsulation-dehydration procedure. Evaluation of genome stability was performed by assessment of amplified fragment length polymorphism (AFLP) markers, which is a PCR- Aug 20, 2014 · We previously reported successful cryopreservation of shoot tips of potato ‘Zihuabai’ by three vitrification-based protocols. In the present study, cryo-injury to shoot tips and genetic stability in regenerants recovered from cryopreserved shoot tips by the three vitrification-based protocols were further investigated.

Abstract. Graduation date: 2007Two studies used DNA markers to assess their usefulness in germplasm\ud identification and evaluation of genetic stability in four cryopreserved Rubus\ud accessions that were stored for over 10 years in liquid nitrogen. Feb 28, 2017В В· evaluated the genetic stability of cryopreserved and non-cryopreserved potato and obtained 97-100% similarity using AFLP markers. Recently, Landey et al . [ 19 ] observed high genetic and epigenetic stability of Coffea arabica plants derived from embryogenic suspensions as well as secondary embryogenesis using AFLP and MSAP markers.

Nov 26, 2016 · Recovery of cryopreserved buds ofthe two purple-fleshed potato by encapsulation-vitrification (Encap-vitri) and droplet-vitrification (Dro-vitri) procedures. Buds of 1.5–2.0 mm and 1.0–1.4 mm that were excised from ‘E03-2677’ and ‘Blue Congo’, were used for Encap-vitri and Drop-vitri cryopreservation, respectively. Genetic and epigenetic stability of cryopreserved and cold-stored hops (Humulus lupulus L.)q Elena L. Peredoa,*, Rosa Arroyo-Garcíab, Barbara M. Reedc, M. Ángeles Revillaa a Department of Plant Physiology, Universidad de Oviedo, Catedrático Rodrigo Uría s/n, 33071 Oviedo, Spain bDepartamento de Biotecnología, Instituto Nacional de Investigación Agraria y Alimentaria, Carretera de A

Attribute Name Values; Creator: Castillo, Nina Rosa F. Abstract: Two studies used DNA markers to assess their usefulness in germplasm identification and evaluation of genetic stability in four cryopreserved Rubus accessions that were stored for over 10 years in liquid nitrogen. Description of the 14 SSR markers used to assess the genome integrity of Solanum lycopersicum Mill. plants from non-cryopreserved and cryopreserved seeds. For each marker, the respective name, forward (Fw) and reverse (Rv) primer sequences, repeat motifs, …

Seedling Development and Evaluation of Genetic Stability. Feb 28, 2017В В· evaluated the genetic stability of cryopreserved and non-cryopreserved potato and obtained 97-100% similarity using AFLP markers. Recently, Landey et al . [ 19 ] observed high genetic and epigenetic stability of Coffea arabica plants derived from embryogenic suspensions as well as secondary embryogenesis using AFLP and MSAP markers., The aim of this study was to compare the genetic stability of chrysanthemum (cv. Pasodoble) apices cryopreserved using two different methods: encapsulation-dehydration and vitrification. These methods were chosen as they are widely used, their efficiency has been reported in several chrysanthemum cultivars, and Me 2 SO is used in one of them..

DIGITAL.CSIC Cryopreservation of somatic embryos of Alnus

evaluation of genetic stability in cryopreserved solanum pdf

Genetic Stability Assessment of in Vitro Plants by. Apr 03, 2014В В· Genetic evaluation of mesenchymal stem cells by G-banded karyotyping in a Cell Technology Center. since the maintenance of a normal karyotype is a reliable indicator of genetic stability of MSCs13 and must be considered as a release criterion for the clinical use of these cells.14., Sep 01, 2008В В· Read "Assessment of genetic and epigenetic changes following cryopreservation in papaya, Plant Cell Reports" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips..

Evaluation of genetic stability in cryopreserved Solanum. Sep 01, 2008 · Read "Assessment of genetic and epigenetic changes following cryopreservation in papaya, Plant Cell Reports" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips., PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF PLANTS REGENERATED FROM NON-CRYOPRESERVED AND CRYOPRESERVED WILD SOLANUM LYCOPERSICUM MILL. SEEDS Byron Zevallos1, Inaudis Cejas2, Florent Engelmann3, Domenico Carputo4, Riccardo To evaluate the genetic stability ….

DIGITAL.CSIC Cryopreservation of somatic embryos of Alnus

evaluation of genetic stability in cryopreserved solanum pdf

Evaluation of genetic stability in cryopreserved Prunus. The evaluation of the genetic stability of Prunus Ferlenain plants regenerated from cryopreserved apices was investigated. cations in cryopreserved explants and regenerated plants [20]. It is thus necessary to verify that genetic stability of cryopreserved material is not altered before routinely using this technique for long-term conservation of plant genetic resources [21]. There are very few reports of phenotypic variations occurring during . in vitro.

evaluation of genetic stability in cryopreserved solanum pdf


Genetic and epigenetic stability of cryopreserved and cold-stored hops (Humulus lupulus L.)q Elena L. Peredoa,*, Rosa Arroyo-Garcíab, Barbara M. Reedc, M. Ángeles Revillaa a Department of Plant Physiology, Universidad de Oviedo, Catedrático Rodrigo Uría s/n, 33071 Oviedo, Spain bDepartamento de Biotecnología, Instituto Nacional de Investigación Agraria y Alimentaria, Carretera de A Jan 15, 2011 · Genetic stability in chrysanthemum (cultivar ‘Pasodoble’) apices was studied at each step of an encapsulation–dehydration cryopreservation protocol: control shoots (A), nodal segments after cold treatment (N), apices after osmotic stress (0.3 M sucrose) and cold treatment (P), encapsulation and culture in 0.8 M sucrose (S), dehydration (D), and

Feb 28, 2017В В· evaluated the genetic stability of cryopreserved and non-cryopreserved potato and obtained 97-100% similarity using AFLP markers. Recently, Landey et al . [ 19 ] observed high genetic and epigenetic stability of Coffea arabica plants derived from embryogenic suspensions as well as secondary embryogenesis using AFLP and MSAP markers. Abstract Questions often arise concerning the genetic stability of plant materials stored in liquid nitrogen for long time periods. This study examined the genetic stability of cryopreserved shoot tips of Rubus germplasm that were stored in liquid nitrogen for more than 12 yr, then rewarmed and regrown. We analyzed the genetic stability of Rubus

The use of mesenchymal stem cells/mesenchymal stromal cells (MSCs) in regenerative medicine is increasing throughout the world. These cells can easily be isolated from different tissues and expanded in vitro to produce a sufficient number of cells that do not have infusion-related toxicity in humans Thus, it is important to mon- of article leads us to think about some specific issues about itor the integrity of MSCs before their clinical use.2,6–10 Other the genetic evaluation of MSCs and the need for discussion, researchers did not find genetic alterations and say …

The aim of this study was to compare the genetic stability of chrysanthemum (cv. Pasodoble) apices cryopreserved using two different methods: encapsulation-dehydration and vitrification. These methods were chosen as they are widely used, their efficiency has been reported in several chrysanthemum cultivars, and Me 2 SO is used in one of them. Cryopreservation did not affect the plant regeneration potential of A. glutinosa through somatic embryogenesis. The genetic stability of the regenerated material was assessed by flow cytometry. Analysis of DNA ploidy stability of the control, PVS2 treated, cryopreserved somatic embryos and plantlets showed no significant differences. URI:

Evaluation of genetic stability in cryopreserved give us a preliminary information on the genetic prunus. In Vitro Cell Developmental Biology Plant 38: stability of the wild pear cryopreserved material 493–500. and let us to be conident of the possibility of using Kawiak, A. & Lojkowska, E. 2004. Thus, it is important to mon- of article leads us to think about some specific issues about itor the integrity of MSCs before their clinical use.2,6–10 Other the genetic evaluation of MSCs and the need for discussion, researchers did not find genetic alterations and say …

Jan 15, 2011 · Genetic stability in chrysanthemum (cultivar ‘Pasodoble’) apices was studied at each step of an encapsulation–dehydration cryopreservation protocol: control shoots (A), nodal segments after cold treatment (N), apices after osmotic stress (0.3 M sucrose) and cold treatment (P), encapsulation and culture in 0.8 M sucrose (S), dehydration (D), and Nov 15, 2013 · Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum .

cations in cryopreserved explants and regenerated plants [20]. It is thus necessary to verify that genetic stability of cryopreserved material is not altered before routinely using this technique for long-term conservation of plant genetic resources [21]. There are very few reports of phenotypic variations occurring during . in vitro Jan 15, 2011 · Genetic stability in chrysanthemum (cultivar ‘Pasodoble’) apices was studied at each step of an encapsulation–dehydration cryopreservation protocol: control shoots (A), nodal segments after cold treatment (N), apices after osmotic stress (0.3 M sucrose) and cold treatment (P), encapsulation and culture in 0.8 M sucrose (S), dehydration (D), and

JJBS Volume 10, Number 1,March 2017 ISSN 1995-6673 Pages 19 - 28 Jordan Journal of Biological Sciences Cryopreservation of Thymbra spicata L. var. spicata and Genetic Stability Assessment of the Cryopreserved Shoot Tips after Feb 28, 2017В В· evaluated the genetic stability of cryopreserved and non-cryopreserved potato and obtained 97-100% similarity using AFLP markers. Recently, Landey et al . [ 19 ] observed high genetic and epigenetic stability of Coffea arabica plants derived from embryogenic suspensions as well as secondary embryogenesis using AFLP and MSAP markers.

PHENOTYPIC AND MOLECULAR CHARACTERIZATION OF PLANTS REGENERATED FROM NON-CRYOPRESERVED AND CRYOPRESERVED WILD SOLANUM LYCOPERSICUM MILL. SEEDS Byron Zevallos1, Inaudis Cejas2, Florent Engelmann3, Domenico Carputo4, Riccardo To evaluate the genetic stability … the genetic stability of potato (Solanum tuberosum) in vitro plants regenerated from apices cryopreserved either by the slow freezing procedure or by the encapsulation-dehydration procedure. Evaluation of genome stability was performed by assessment of amplified fragment length polymorphism (AFLP) markers, which is a PCR-

Summary. The evaluation of the genetic stability of Prunus Ferlenain plants regenerated from cryopreserved apices was investigated. The analysis of plants recovered from frozen material was performed at the phenotypic (developmental competence), cytological (chromosomal preparations) and molecular level [random amplified polymorphic DNA (RAPD)... Cryopreservation did not affect the plant regeneration potential of A. glutinosa through somatic embryogenesis. The genetic stability of the regenerated material was assessed by flow cytometry. Analysis of DNA ploidy stability of the control, PVS2 treated, cryopreserved somatic embryos and plantlets showed no significant differences. URI:

The use of mesenchymal stem cells/mesenchymal stromal cells (MSCs) in regenerative medicine is increasing throughout the world. These cells can easily be isolated from different tissues and expanded in vitro to produce a sufficient number of cells that do not have infusion-related toxicity in humans Analyses of ploidy and molecular stability of plantlets recovered from cryopreserved rhizome buds of two selected genotypes showed no differences compared with the mother plants. Cryopreservation of RB explants of A. officinalis with this Encapsulation–Dehydration procedure will be useful in long-term preservation programs.

Apr 03, 2014 · Genetic evaluation of mesenchymal stem cells by G-banded karyotyping in a Cell Technology Center. since the maintenance of a normal karyotype is a reliable indicator of genetic stability of MSCs13 and must be considered as a release criterion for the clinical use of these cells.14. Jan 15, 2011 · Genetic stability in chrysanthemum (cultivar ‘Pasodoble’) apices was studied at each step of an encapsulation–dehydration cryopreservation protocol: control shoots (A), nodal segments after cold treatment (N), apices after osmotic stress (0.3 M sucrose) and cold treatment (P), encapsulation and culture in 0.8 M sucrose (S), dehydration (D), and

Abstract. Questions often arise concerning the genetic stability of plant materials stored in liquid nitrogen for long time periods. This study examined the genetic stability of cryopreserved shoot tips of Rubus germplasm that were stored in liquid nitrogen for more than 12 yr, then rewarmed and regrown. Genetic stability of wild pear (Pyrus pyraster, Burgsd) after cryopreservation by encapsulation dehydration described for cryopreservation of Solanum shoot tips (Fabre and Dereuddre 1990), has been also success- The present study evaluates the genetic stability in wild pear cryopreserved …

Analyses of ploidy and molecular stability of plantlets recovered from cryopreserved rhizome buds of two selected genotypes showed no differences compared with the mother plants. Cryopreservation of RB explants of A. officinalis with this Encapsulation–Dehydration procedure will be useful in long-term preservation programs. Shoot tips of Dioscorea floribunda, a medicinal species of yam, were cryopreserved using the vitrification technique, resulting in 87% survival and 30% plant regeneration.Genetic stability of plants derived from cryopreserved shoot tips was evaluated using molecular, morphological and biochemical methods.

Abstract. Graduation date: 2007Two studies used DNA markers to assess their usefulness in germplasm\ud identification and evaluation of genetic stability in four cryopreserved Rubus\ud accessions that were stored for over 10 years in liquid nitrogen. In the present investigation, the genetic stability of potato (Solanum tuberosum L.) plantlets of the cultivars Agria and Marphona stored under cryopreservation and non-cryopreservation conditions

Genetic stability of the regenerated material was assessed by flow cytometry (FCM), amplified fragment length polymorphisms (AFLP) and simple sequence repeats (SSR). Cryopreservation of embryogenic clusters involved encapsulation of each clusterin an alginate bead, followed by a 3-day culture in 0.7 M sucrose and subsequent desiccation to 25 or cations in cryopreserved explants and regenerated plants [20]. It is thus necessary to verify that genetic stability of cryopreserved material is not altered before routinely using this technique for long-term conservation of plant genetic resources [21]. There are very few reports of phenotypic variations occurring during . in vitro

Nov 15, 2013В В· Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum . Attribute Name Values; Creator: Castillo, Nina Rosa F. Abstract: Two studies used DNA markers to assess their usefulness in germplasm identification and evaluation of genetic stability in four cryopreserved Rubus accessions that were stored for over 10 years in liquid nitrogen.

Dec 01, 2010 · Read "Fertile plant regeneration from cryopreserved calli of Oryza rufipogon Griff. and assessment of variation in the progeny of regenerated plants, Plant Cell Reports" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at … Genetic stability of the regenerated material was assessed by flow cytometry (FCM), amplified fragment length polymorphisms (AFLP) and simple sequence repeats (SSR). Cryopreservation of embryogenic clusters involved encapsulation of each clusterin an alginate bead, followed by a 3-day culture in 0.7 M sucrose and subsequent desiccation to 25 or

Genetic Stability of Cryopreserved Grapevine (Vitis vinifera L.) Genome by Vitrification Method 382 month in LN (T7). After each treatment, freezing buds were thawed in a water-bath at 38 В°C for 3 min. Summary. The evaluation of the genetic stability of Prunus Ferlenain plants regenerated from cryopreserved apices was investigated. The analysis of plants recovered from frozen material was performed at the phenotypic (developmental competence), cytological (chromosomal preparations) and molecular level [random amplified polymorphic DNA (RAPD)...